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Rabbit Anti-Nestin antibody (bs-0008R)
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说明书: 50ul  100ul  200ul
20ul/380.00元
50ul/780.00元
100ul/1380.00元
200ul/2200.00元
大包装/询价

产品编号 bs-0008R
英文名称 Nestin
中文名称 巢蛋白/神经上皮干细胞蛋白抗体
别    名 ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin.   
Specific References  (3)     |     bs-0008R has been referenced in 3 publications.
[IF=3.17] Liu, Yang, et al. "Amelioration of Stroke-Induced Neurological Deficiency by Lyophilized Powder of Catapol and Puerarin." International Journal of Biological Sciences 10.4 (2014): 448-456.  IHC-P ;  Mouse.  
[IF=1.64] Shan, Tengfei, et al. "Lithium chloride promotes the odontoblast differentiation of hair follicle neural crest cells by activating Wnt/β‐catenin signaling." Cell Biology International (2014).  IF(ICC) ;  Mouse.  
[IF=0.97] Kawashima, Satoki, Koichi SHINKAI, and Masaya SUZUKI. "Effect of an experimental adhesive resin containing multi-ion releasing fillers on direct pulp-capping." Dental Materials Journal 35.3 (2016): 479-489.  IHC-P ;  Rat.  
规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 50ul  100ul  200ul
研究领域 肿瘤  心血管  发育生物学  神经生物学  干细胞  细胞类型标志物  肿瘤细胞生物标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse, Rat, 
产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test  IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 209kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from rat Nestin:681-750/1893 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.

Subcellular Location:
cytoplasm

Tissue Specificity:
CNS stem cells.

Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Similarity:
Belongs to the intermediate filament family.

SWISS:
P21263

Gene ID:
25491

Database links:

Entrez Gene: 10763 Human

Entrez Gene: 18008 Mouse

Entrez Gene: 25491 Rat

Omim: 600915 Human

SwissProt: P48681 Human

SwissProt: Q6P5H2 Mouse

SwissProt: P21263 Rat

Unigene: 527971 Human

Unigene: 331129 Mouse

Unigene: 9701 Rat




Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.


神经干细胞标志物


在动物体早期发育过程中,中枢神经系统发育尤为重要 。Nestin是一种中等纤维蛋白,它在哺乳动物神经前体细胞中表达较高,目前已被广泛用于神经前体细胞的重要标志分子。研究Nestin的表达调控规律,将有助于人们认识神经前体细胞的生物特性。此抗体还表达中枢神经系统干细胞及神经上皮细胞。
产品图片
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nestin Polyclonal Antibody, Unconjugated(bs-0008R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Cell: Neuro-2a
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control on Neuro-2a(blue) compared with primary antibody(Cat#: bs-0008R)(green) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
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