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Rabbit Anti-SREBP1 antibody (bs-1402R)
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说明书: 50ul  100ul  200ul
20ul/380.00元
50ul/780.00元
100ul/1380.00元
200ul/2200.00元
大包装/询价

产品编号 bs-1402R
英文名称 SREBP1
中文名称 胆固醇调节元件结合蛋白1抗体
别    名 Sterol regulatory element binding protein 1; ADD 1; BHLHD1; D630008H06; SREBF 1; SREBP-1; SREBF1; SREBP 1; SREBP1; SREBP 1c; Sterol regulatory element binding protein 1; Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1; Sterol regulatory element binding transcription factor 1; SRBP1_HUMAN; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1; Processed sterol regulatory element-binding protein 1.  
Specific References  (1)     |     bs-1402R has been referenced in 1 publications.
[IF=7.67] Zhang, Xian, et al. "ROS-induced TXNIP drives fructose-mediated hepatic inflammation and lipid accumulation through NLRP3 inflammasome activation."Antioxidants and Redox Signaling ja (2015).  WB ;  Rat.  
规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 50ul  100ul  200ul
研究领域 心血管  转录调节因子  合成与降解  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 54/126kDa
细胞定位 细胞核 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SREBP-1:301-450/1147 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').

Subunit:
Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA.

Subcellular Location:
Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Processed sterol regulatory element-binding protein 1: Nucleus.
Isoform SREBP-1aDelta: Nucleus.
Isoform SREBP-1cDelta: Nucleus.

Tissue Specificity:
Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.

Post-translational modifications:
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding.

Similarity:
Belongs to the SREBP family.
Contains 1 bHLH (basic helix-loop-helix) domain.

SWISS:
P36956

Gene ID:
6720

Database links:

Entrez Gene: 6720 Human

Entrez Gene: 20787 Mouse

Entrez Gene: 78968 Rat

Omim: 184756 Human

SwissProt: P36956 Human

SwissProt: Q9WTN3 Mouse

SwissProt: P56720 Rat

Unigene: 592123 Human

Unigene: 278701 Mouse

Unigene: 296366 Mouse

Unigene: 198857 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

转录调节因子(Transcriptin Regulators)
胆固醇调节元件结合蛋白1(SREBP1)是膜结合的转录因子,参与脂质自稳态的许多作用(胆固醇、脂肪酸、甘油三酯和磷酯的合成/摄取).SREBP1在内质网上合成,需要被转运到高尔基体并经过一系列蛋白水解后才能到核内发挥作用。
未成熟时在胞浆表达,水解后的成熟片段在胞核表达。
产品图片
Sample:
Liver(Mouse) Cell Lysate at 40 ug
Liver(Rat) Cell Lysate at 40 ug
Primary: Anti-SREBP1 (bs-1402R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54/126 kD
Observed band size: 54 kD
Sample: Muscle(Mouse) Lysate at 30 ug
Primary: Anti- SREBP1 (bs-1402R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54/126 kD
Observed band size: 50 kD
Sample:
Lung(Mouse) Lysate at 30 ug
Ovary (Mouse) Lysate at 30 ug
Skin (Mouse) Lysate at 30 ug
Primary: Anti- SREBP1 (bs-1402R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54/126 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (bs-1402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (bs-1402R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (bs-1402R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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