客服热线:400-901-9800          客服QQ:4009019800          技术答疑     技术支持     质量反馈     联系我们
Rabbit Anti-MAGEB3 antibody (bs-6823R)
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购传真:010-58129612
技术支持:support@bioss.com.cn
说明书: 100ul  200ul
100ul/1380.00元
200ul/2200.00元
大包装/询价

产品编号 bs-6823R
英文名称 MAGEB3
中文名称 黑色素瘤相关抗原B3抗体
别    名 Cancer/testis antigen family 3 member 5; CT3.5; MAGB3_HUMAN; MAGE B3 antigen; MAGE-B3 antigen; MAGEB3; melanoma antigen family B, 3; Melanoma associated antigen B3; Melanoma-associated antigen B3.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 肿瘤  细胞生物  免疫学  细胞类型标志物  肿瘤细胞生物标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Horse, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 39kDa
细胞定位 细胞核 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MAGEB3:201-300/346 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
MAGEB3 is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region. It is expressed in testis and placenta, and in a significant fraction of tumors of various histological types. The MAGEB genes are clustered on chromosome Xp22-p21.

Tissue Specificity:
Expressed in testis.

Similarity:
Contains 1 MAGE domain.

SWISS:
O15480

Gene ID:
4114

Database links:

Entrez Gene: 4114 Human

Omim: 300152 Human

SwissProt: O15480 Human

Unigene: 113290 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti- MAGEB3 (bs-6823R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
Tissue/cell: mouse testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MAGEB3 Polyclonal Antibody, Unconjugated(bs-6823R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Human melanoma tumor; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MAGEB3 Polyclonal Antibody, Unconjugated(bs-6823R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MAGEB3 Polyclonal Antibody, Unconjugated(bs-6823R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
版权所有 2016-2020 www.bioss.com.cn 注册送38体验金-注册送28元体验金app|注册送体验金娱乐游戏 京ICP备05066980号 京公网安备110107000727号
通过国际质量管理体系ISO9001:2008 GB/T19001-2008认证    编号:00115Q211807R1M/1100